Electrochemical Characterization of Metal-Binding properties of Metallothioneins Isolated from M.f galloprovincialis

摘要

Amperometric titrations of defined metallothionein concentrations with the standard CdCl_2 solution have been performed in seawater samples at pH≈1 and pH≈8, in order to define the metal-binding properties of purified mussel metallothionein component. The con- centration of the formed Cd-thionein complex ahs been assessed in the indirect and direct mode. The results on the available ligand concentration C_L for complexing Cd~2+ ions and the apparent sta- bility constant K, determined by two procedures are compared and the differences discussed.