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Isolation and characterization of fourteen microsatellite loci for striped field mouse (Apodemus agrarius)

机译:条纹田鼠(Apodemus agrarius)的14个微卫星基因座的分离和鉴定

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摘要

We describe the isolation and characterization of 14 microsatellite loci in the striped field mouse from genomic DNA-enriched libraries in this paper. The 14 new loci were tested in 24 individuals from four populations in Southwest China. These loci were highly polymorphic with numbers of alleles per locus ranging from 6 to 14 and expected heterozygosities from 0.789 to 0.925. All loci followed Hardy–Weinberg expectations except SFM4, SFM11 and SFM13 loci. No significant linkage association was found among all loci. The 14 novel polymorphic microsatellite loci will be useful in studying phylogeography and population genetics of the striped field mouse.
机译:我们在本文中描述了从富含基因组DNA的文库中的条带化田鼠中14个微卫星基因座的分离和表征。在来自中国西南部四个人口的24个人中测试了这14个新基因座。这些基因座是高度多态的,每个基因座的等位基因数量为6至14,预期杂合度为0.789至0.925。除SFM4,SFM11和SFM13基因座外,所有基因座均遵循Hardy–Weinberg的期望。在所有基因座中均未发现明显的连锁关联。 14个新的多态微卫星基因座将对研究条纹田鼠的系统地理学和种群遗传学很有用。

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  • 来源
    《Conservation Genetics》 |2008年第6期|1691-1693|共3页
  • 作者单位

    Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Datun Road Chaoyang District Beijing 100101 People’s Republic of China;

    Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Datun Road Chaoyang District Beijing 100101 People’s Republic of China;

    Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Datun Road Chaoyang District Beijing 100101 People’s Republic of China;

    Sichuan Academy of Forestry Chengdu 610066 Sichuan People’s Republic of China;

    Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Datun Road Chaoyang District Beijing 100101 People’s Republic of China;

    Institute of Rare Animals and Plants China West Normal University Nanchong 637002 Sichuan People’s Republic of China;

    Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Datun Road Chaoyang District Beijing 100101 People’s Republic of China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Striped field mouse (Apodemus agrarius); Microsatellites; Heterozygosity; Phylogeography; Population genetics;

    机译:条纹田鼠(Apodemus agrarius);微卫星;杂合性;体谱学;种群遗传学;

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