Analytical and Biological Evaluation of High Throughput Screen Actives Using Evaporative Light Scattering, Chemiluminescent Nitrogen Detection, and Accurate Mass LC-MS-MS

摘要

In this investigation the utility of evaporative light scattering detection (ELSD) combined withnHPLC-MS was demonstrated as a key component of a bioassay-guided fractionation, or “biofractionation”ntechnique, for the evaluation of high throughput screen actives. ELSD provided on-line analyte massninformation that was critical for the classification of the samples. Chemiluminescent nitrogen detectionn(CLND) was also evaluated for sample concentration estimation for nitrogen-containing compounds, andnaccurate mass LC-MS-MS analysis was employed for rapid structural confirmation and elucidation ofncomponents previously identified as active via biofractionation.