CE Determination of Monosaccharides in Pulp Using Indirect Detection and Curve-Fitting

摘要

In the present work a capillary electrophoretic method for the analysis of monosaccharides utilizing indirect UV-detection has been developed. Different probes for indirect detection have been assessed using model carbohydrate samples. Background electrolytes with or without addition of cetyltrimethylammonium bromide have also been evaluated regarding the separation power. Furthermore, a curve-fitting algorithm has been introduced to increase the separation resolution. The optimized method has been used for analysis of monosaccharides from an acidically hydrolyzed pulp sample.