Identification and analysis of ARS function of six plant MARs

摘要

Six plant MARs isolated from tobacco and Arabdiposis were investigated for their ARS activity in yeast. The results showed that among the six plant MARs, onh TM1 and AM4 had strong ARS activity which was almost the same as that of ARS from yeast chromosome. In order to further identify the core region of the two MARs for the ARS activity, a series of subciones were created by PCR strategy, and the corresponding subciones were designated as TM1-1, TM1-2, TM1-3, AM4-1, AM4-2 and AM4-3. respectively. Our studies revealed that TM1-3 and AM4-3 not only had higher ARS activity, but also displayed higher transformation frequency, plasmid stability and growth rate compared to their intact MARs, TM1 and AM4. These data present an important clue for further elucidating the relationship between MAR and ARS.