P53 gene mutations in non-small cell lung cancer detected by polymerase Chain reaction single-strand conformation polymorphism analysis

摘要

Mutations of the p53 tumor suppressor gene are the most frequent genetic alterations detected in hu- man lung cancer. To assess the pathogenic significance of p53 gene alterations in Chinese non-small cell lung cancer(NSCLC), 74 paired samples of primary lung cancer and normal lung tissue far away from the cancer were analyzed for multations of the p53 gene(exons 5-8)using exon-specific PCR, single-strand con- formation polymorphism(PCR-SSCP). p53 mutations were observed in 55.4/100(41/74)of the samples.