Effect of mitochondrial K_(ATP) channel on voltage-gated K~+ channel in 24 hour-hypoxic human pulmonary artery smooth muscle cells

摘要

Background Hypoxic pulmonary hypertension (HPH) is initiated by inhibition of O_2-sensitive, voltage-gated (Kv) channels in pulmonary arterial smooth muscle cells (PASMCs). The mechanism of hypoxic pulmonary hypertension has not yet been fully elucidated. The mitochondrial ATP-sensitive K~+ channel ( MitoK_(ATP) ) is extremely sensitive to hypoxia, and is a decisive factor in the control of mitochondrial membrane potential ( ΔΨ_m) . This study investigated the changes of cell membrane potential and Kv channel in cultured human pulmonary artery smooth muscle cell ( hPASMC ) exposed to 24 hour-hypoxia , and explored the role of MitoK_(ATP) and ΔΨ_m in this condition. Methods Fresh human lung tissues were obtained from the patients undergoing a chest operation. hPASMCs were isolated, cultured, and divided into 6 groups: ① control group, cultured under normoxia; ② diazoxide group, cultured in normoxia with diazoxide, an opener of MitoK_(ATP); ③ 5-HD group, cultured in normoxia with sodium 5-hydroxydecanoate (5-HD), an antagonist of MitoK_(ATP); ④ 24 hour-hypoxia group; ⑤ 24 hour-hypoxia + diazoxide group; and ⑥ 24 hour-hypoxia + 5HD group. Whole-cell patch-clamp technique was used to trace the cell membrane K~+ currents. The expressions of cell membrane Kv1.5 mRNA and protein were determined by RT-PCR and Western blot technique, respectively. The relative changes in mitochondrial potential were tested with rhodamine fluorescence (R-123) technique. Results After exposure to diazoxide for 24 hours, the intensity of R-123 fluorescence in normoxic hPASMCs was significantly increased compared with control group ( P < 0.05 ) , but there were no significant changes in these tests after the hPASMCs had been exposed to 5-HD for 24 hours. Twenty-four hour-hypoxia or 24 hour-hypoxia + diazoxide could markedly increase the intensity of R-123 fluorescence in hPASMC and the changes were more significant in 24 hour-hypoxia + diazoxide group than in 24 hour-hypoxia group ( P < 0.05 ) although 5-HD could partly weaken the effect of 24 hour-hypoxia on the intensity of R-123 fluorescence. After exposure to diazoxide for 24 hours, the cell membrane K~+ currents and the expression of cell membrane Kvl. 5 mRNA and protein in normoxic hPASMCs were significantly decreased compared with control group ( P < 0.05 ) , but there were no significant changes in these tests after the hPASMCs had been exposed to 5-HD for 24 hours. Also, 24 hour-hypoxia or 24 hour-hypoxia + diazoxide decreased the cell membrane K~+ currents and the expression of Kv1.5 mRNA and protein (P < 0.05) but the changes were more significant in 24 hour-hypoxia + diazoxide group than in 24 hour-hypoxia group ( P < 0.05 ). Again, 5-HD could partly weaken the inhibitory effect of 24 hour-hypoxia on the cell membrane K~+ currents and the expression of Kv1.5 mRNA or protein (P < 0.05). Conclusions The opening of MitoK_(ATP) followed by a depolarization of ΔΨ_m in hypoxia might contribute to the alterations in the expression of cell membrane Kv1.5 mRNA and protein leading to change in the cell membrane potential of hypoxic hPASMCs. This might be a mechanism of the development of hypoxic pulmonary hypertension.