Direct detection of oxygen free radicals produced in the viscera of burned rats using electron paramagnetic resonance spectroscopy

摘要

Objective: To detect superoxide anion (O_2~-) signals in the heart, liver, lung and kidney tissues after burns. Methods: Twenty-four male rats were randomized into 4 groups. The rats of experimental groups were immersed in 100℃ water for 15 seconds and 25% third-degree burn was created. Thoracotomy or laparotomy was performed at 5, 10, 15, 20 and 30 minutes after burn, and specimens of the heart, lung, liver kidney were obtained for burned rats. The specimens were then preserved in liquid nitrogen for cryo-preservation and detected immediately using EPR. Results: The signals of superoxide O radical appeared in the heart, lung, liver and kidney specimens 10-15 minutes after burn. Conclusions: There is a direct evidence of oxygen free radicals (OFRs) injury to viscera of burned rats between 10-15 minutes after burn.