Selective Affinity Separation of Yeast Alcohol Dehydrogenase by Reverse Micelles with Unbound Triazine Dye

摘要

The reversed micelles were formed with cationic cetyltrimethylammonium bromide (CTAB) as surfa- tant and n-hexanol as cosolvent in the CTAB (50 mmol.L~-1)/hexanol (15/100 by volume)/hexane system. Cibacron Blue 3GA (CB) as an affinity ligand in the aqueous phase was directly introduced to the reversed micelles with Electrostatic interaction between anionic CB and cationic surfactant.