DNA Content and Gene Expression in Adenoma-Carcinoma Sequence of Gallbladder Carcinogenesis

摘要

Objective To study the proliferative change of epithelium and gene expression in adenoma-carcinoma sequence of gallbladder carcinogenesis. Methods Mutations of K-ras gene and APC gene were detected by PCR-RFLP, and the expression of p53 protein was detected by ABC immunohistoehemistry. DNA contents were detected by using a TJTY-300 automatic image analyzer. Results Cell DNA contents and atypia were increased gradually in the adenoma-carcinoma sequence of gallbladder. Aneuploidy in gallbladder adenoma was associated with the size of adenoma. When the diameter of adenoma was more than 1 cm, the aneuploidy was significantly increased (P<0.05). APC gene and K-ras gene mutations were detected in the adenoma-carcinoma pathway. And p53 protein overexpression was not detected in this pathway. Conclusion Adenoma-carcinoma sequence pathway plays an important role in gallbladder carcinogenesis. Gallbladder adenoma has an obvious tendency to malignant transformation, especially in the patients with adenoma of 1 cm in diameter or above. It has molecular abnormalities different from those de novo carcinomas.