A comparative study of changing patterns of con-canavalin A-binding proteins in early stage of cholesterol gallstone

摘要

AIM To elucidate the importance and the changing patterns of biliary concanavalin A-binding proteins (CPs) in the early stage of cholesterol gallstone. METHODS CPs concentrations and nucleation activities were measured by lectin affinity chromatography in biles of patients with cholesterol gallstone, pigment gallstone, gallbladder cholesterosis and non-biliary diseases. RESULTS The concentrations of CPs were much higher in patients with cholesterol gallstone (0.39 g/L ±0.11 g/L, n = 36, P < 0.01) or gallbladder cholesterosis (0.40 g/L±0.09 g/L, n = 9, P < 0.01) than in those with pigment gallstone (0.26 g/L±0.12 g/L, n = 7) and/or non-biliary diseases (0.27 g/L±0.09 g/ L, n = 10). Pronucleating activities were much stronger in patients with cholesterol gallstones (nucleation time ratio: 0.57 ±0.21, n = 5, P < 0.01 vs pigment gallstone and/or non-biliary diseases) and gallbladder cholesterosis (nucleation time ratio: 0.44 ± 0.23, n=5, P < 0.01 vs pigment gallstone or non-bil-liary diseases). The binding percentages of CPs to model biliary vesicles were also higher in patients with cholesterol gallstones (n=6) than those with pigment gallstones (n = 6) (2.4% ±0.9% vs 0.9% ±0.5%, P < 0.01). CONCLUSION Hypersecretion of CPs, especially those in vesicular phase may be the important changes in the early stage of cholesterol gallstone.