Impact of exposure of crude oil and dispersant (COREXIT~® EC 9500A) on denitrification and organic matter mineralization in a Louisiana salt marsh sediment

摘要

In response to the 2010 oil spill from the explosion of the Deepwater Horizon oil rig in the Gulf of Mexico, this experiment aims to study the ecological impact of the crude oil and dispersant (COREXIT~® EC 9500A) in a coastal salt marsh ecosystem. The marsh sediment was incubated under an anaerobic condition with exposure to the crude oil or/and dispersant. The experiments were conducted in two continuous phases of nitrate addition to study denitrification potential using acetylene blockage technique and organic matter mineralization potential indicated by CO_2 production in the sediment. Results show that the oil slightly (with no statistical significance p ＞ 0.05) increased both the denitrification and organic matter mineralization activities, likely due to oil components serving as additional organic matter. In contrast, the dispersant significantly (p ＜ 0.05) inhibited denitrification, but stimulated organic matter mineralization activities in the sediment due to unknown mechanisms. As a consequence, redox potentials (Eh) were much lower in the dispersant treated systems. The ecological impacts from the dispersant exposure may come from two fronts. First, loss of organic matter from the coastal marsh will threaten the long-term stability of the ecosystem, and the decrease in denitrification activity will weaken the N removal efficiency. Secondly, more reducing conditions developed by the dispersant exposure will likely preserve the oil in the ecosystem for an extended period of time due to weaker oil biodegradation under anaerobic conditions.