Drinking water disinfection byproduct iodoacetic acid interacts with catalase and induces cytotoxicity in mouse primary hepatocytes

摘要

Disinfection byproducts (DBPs) are produced during the disinfection of drinking water and pose a hazard to human health. As a typical type of DBPs, iodoacetic acid (IAA) exhibits prominent cytotoxicity in mammalian cell systems which links with oxidative stress. However, little is known about the relationship of catalase (CAT) with the cytotoxicity of IAA and the adverse effects of IAA to CAT. This study investigated the effects of IAA on the cell viability and CAT activity in the mouse primary hepatocytes. It was shown that IAA exposure induced the loss of cell viability and the increase of intracellular CAT activity. Intracellular CAT activity significantly increased due to the stimulation of CAT production under IAA exposure. The molecular CAT activity was inhibited due to the direct interaction of IAA with HIS 74 and TYR 357 around the active sites of CAT. IAA binds to CAT with (4.05 +/- 1.98) sites via van der Waals and hydrogen bonding interactions, resulting in the loosening of protein skeletons and the change of protein size. (C) 2018 Elsevier Ltd. All rights reserved.