Aptamer/Nanoparticle-Based Sensitive, Multiplexed Electronic Coding of Proteins and Small Biomolecules through a Backfilling Strategy

摘要

Sensing large and small simultaneously: Sensitive and multiplexed analysis of lysozyme and ATP analytes is achieved through target-induced release of aptamers from the DNA duplexes on a sensing surface, followed by backfilling hybridization of the resulting single-stranded DNAs with aptamers conjugated to the electrochemically encoded nanoparticles (see graphic).