DEVELOPMENT OF A MICROFLUIDIC DEVICE USING NANOPARTICLE-BASED BIO-BARCODES FOR ULTRASENSITIVE DETECTION OF PROTEINS

摘要

We present a microfluidic system that allows for ultra-sensitive protein detection. The system integrates the bio-bar-code amplification (BCA) [1] approach with silver staining on a single microfluidic device to detect Prostate Specific Antigen in concentrations as low as 500 attomolar (300 proteins in the entire sample), using several orders of magnitude less sample volume than necessary for the bench-top assay.