Smart Trypsin Adsorption into N-(2-Aminoethyl)-3-aminopropyl-Modified Mesoporous Silica for Ultra Fast Protein Digestion

摘要

Efficient mesoporous proteolytic networks: N-(2-Aminoethyl)-3-aminopropyl-derivatized mesoporous silica particles, with a 4.4 nm pore diameter, efficiently promote a comfortable entrapment of trypsin into mesoporous networks, thus providing very fast and effective proteolytic activity. The rapid digestion and highly confident protein identification (100 % sequence coverage in just 1 min) offers a practical and convenient alternative to conventional in-solution digestion.