Amplified Fluorescence Determination Of Micrornas In Homogeneous Solution With Cationic Conjugated Polymers

摘要

Combining the miRNA-primed extension reaction and the optical amplification of a conjugated polymer, a sensitive, selective and homogeneous method for miRNA determination has been developed based on a fluorescence resonance energy transfer (FRET) mechanism.rnMicroRNAs (miRNAs) are a class of small (19-23 nucleo-tides), endogenous regulatory RNA molecules, which are believed to be involved in the regulation of nearly 30% of all human genes. Moreover, recent studies have pointed towards a connection between specific changes in miRNA expression and various diseases, especially for various types of cancer. In the past five years there has been a surge of interest in understanding the regulatory roles of miRNA in cellular processes. MiRNA assay plays a critical role in better understanding the biological function of miRNAs and has great potential in early disease diagnostics and new drug discovery.