A New Bio-active Surface For Protein Immobilisation Via Copper-free 'click' Between Azido Sam And Alkynyl Fischer Carbene Complex

摘要

Fischer carbene complex anchored on glass or silicon surface using a Cu-free 'click' reaction allows facile and swift covalent grafting of protein molecules like Bovine Serum Albumin (BSA).rnProtein immobilisation on a surface using covalent linkage is vital to proteome research that includes development of microarray- and biochip-based sensors for high-throughput screening of protein structure and function, study of interaction between protein and other biomolecules, multianalyte immunoassay or enzyme assay for clinical diagnostics, etc. The linkage between the biomolecule and the inorganic solid support remains a critical variable for optimal performance of a sensor device. A wide range of protein immobilisation techniques have been developed and they are continuously being improved. It is well-recognised that a covalent bond between a biomolecule and a functionalised surface lasts longer and provides greater reproducibility than immobilisation obtained by non-covalent links. The covalent bond formation between the anchored protein and the glass surface must be extremely facile and should proceed under ambient conditions to conserve protein/enzyme structure and activity.