Deuterated polymer gels for measuring anisotropic NMR parameters with strongly reduced artefacts

摘要

Perdeuterated poly(styrene) is introduced as an almost artefact-free and arbitrarily scalable alignment medium for measuring residual dipolar couplings and other anisotropic NMR parameters; the spectral quality achievable in this new medium is demonstrated for HSQC spectra leading to the conformational analysis of staurosporine and homonuclear TOCSY-type experiments.rnResidual dipolar couplings (RDCs) as anisotropic NMR-parametcrs play an important role for the structure determination of biomacromolecules and small organic molecules in solution. For the measurement of anisotropic parameters liquid crystalline phases or stretched polymer gels can be used for achieving the necessary partial alignment, with gels having the advantage of arbitrary scalability of the alignment strength. However, alignment media generally introduce undesired NMR-signals which might severely overlap with signals of the molecule of interest and become especially annoying in polymer gels at low alignment strengths.