A temperature independent pH (TIP) buffer for biomedical biophysical applications at low temperatures

摘要

A temperature independent pH buffer has been developed from a combination of buffers of opposite-sign temperature coefficients, and utility in low temperature spectroscopy and storage of pH sensitive compounds is demonstrated. Storage and analysis of samples at low and cryogenic temperatures has become a routine practice in modern research, as these temperatures can preserve the integrity of precious samples, and allow modern biophysical and bioanalytical techniques to provide information on biomolecules at an unprecedented level.