Zn(Ⅱ) complex for selective and rapid scission of protein backbone

摘要

A Zn~Ⅱ complex with an aldehyde group hydrolyzed porcine pancreatic elastase under mild conditions, pH 8.0, 50 ℃, by the Schiff base formation between the Zn~Ⅱ complex and the NH_2 group in the protein, suggesting that a Zn~Ⅱ compound can be active toward peptide hydrolysis when it strongly binds to the substrate.rnZinc is an essential element in living organisms. Enzymes such as carboxypeptidase A, thermolysin, leucine aminopeptidase and a series of matrix metalloproteinases require Zn~Ⅱ in the catalytic center.