Superquenching acridinium ester chemiluminescence by gold nanoparticles for DNA detection

摘要

It was found that the chemiluminescence of acridinium ester (AE) was quenched effectively by gold nanoparticles (AuNPs) with Stern-Volmer constants approaching 10~(10) M~(-1) which was exploited to realize sequence-specific DNA detection based on the preferential absorption of AE-tagged single-strand probe on unmodified AuNPs over the hybrids of probe and target DNA. The inherent sensitivity and simplicity make chemiluminescence (CL) suitable for biosensor miniaturization and integration to develop low cost, portable point-of-need assay systems.