A reverse transcription-free real-time PCR assay for rapid miRNAs quantification based on effects of base stacking

Zhuoxuan Lu; Demin Duan; Rong Cao; Liming Zhang; Kexiao Zheng; Jiong Li

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A reverse transcription-free real-time PCR assay for rapid miRNAs quantification based on effects of base stacking

机译：基于碱基堆积效应的无逆转录实时PCR快速定量miRNA

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摘要

A rapid and reverse transcription-free real-time PCR micro-RNA assay was developed based on effects of base stacking. This microRNA assay has been shown to be highly specific to homogenous miRNAs, and the procedure can be completed within 30 min starting from total RNA.

机译：基于碱基堆积的作用，开发了一种快速且无逆转录的实时PCR微RNA检测方法。已经证明这种microRNA分析对同质miRNA具有高度特异性，并且该过程可以从总RNA开始在30分钟内完成。

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来源
《Chemical Communications》 |2011年第26期|p.7452-7454|共3页
作者
Zhuoxuan Lu; Demin Duan; Rong Cao; Liming Zhang; Kexiao Zheng; Jiong Li;
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Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Dushu Lake Higher Education Town, Suzhou Industrial Park, Suzhou 215123, P. R. China;

Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Dushu Lake Higher Education Town, Suzhou Industrial Park, Suzhou 215123, P. R. China;

Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Dushu Lake Higher Education Town, Suzhou Industrial Park, Suzhou 215123, P. R. China;

Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Dushu Lake Higher Education Town, Suzhou Industrial Park, Suzhou 215123, P. R. China;

Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Dushu Lake Higher Education Town, Suzhou Industrial Park, Suzhou 215123, P. R. China;

Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Dushu Lake Higher Education Town, Suzhou Industrial Park, Suzhou 215123, P. R. China;

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A reverse transcription-free real-time PCR assay for rapid miRNAs quantification based on effects of base stacking

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