Observing ~13C-~13C connectivities at high magnetic fields and very high spinning frequencies

摘要

We report the application of a dipolar recoupling sequence for the observation of ~13C-~13C connectivities in biomolecules at high magnetic fields (B_0> 21.1 T) and ultra-high magic angle spinning frequencies (v_R = 60 kHz). The efficiency and the robustness of this double-quantum technique are demonstrated on the YajG protein (19.6 kDa).