Synthesis of oligodeoxyribonucleotides containing a conformationally-locked anti analogue of O~6-methyl-2'-deoxyguanosine and their recognition by MGMT and Atl1

摘要

We show that DNA containing a conformationally-locked anti analogue of O~6-aIkylguanine is a poor substrate for human O~6-methylguanine-DNA methyltransferase (MGMT) and the alkyltransferase-like protein, Atll. This highlights the requirement for the syn conformation and rationalises why certain O~6-alkylguanines are poor MGMT substrates.