Flagella as a novel alignment medium for the measurement of residual dipolar couplings in proteins

摘要

The two flexible rod-like flagella (～500 nm in diameter and 5-15 nm long) of Chlamydomonas reinhardtii, a unicellular green alga, can weakly align molecules in an external magnetic field, thereby enabling the measurement of various residual dipolar couplings in solution NMR spectroscopy.