Solid phase click ligation for the synthesis of very long oligonucleotides

摘要

Oligonucleotides have been ligated efficiently on solid-phase using Cuaac and SPAAC chemistry to produce up to 186-mer triazole linked DNA products. Multiple sequential ligation reactions can be carried out by using a masked azide approach. This work suggests a novel modular approach to the synthesis of large complex oligonucleotide analogues.