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首页> 外文期刊>Chemical Communications >A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides
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A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides

机译：基于量子点和荧光标记肽之间FRET的蛋白激酶测定

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摘要

A novel protein kinase assay was developed, based on FRET between QDs and fluorescently-labeled substrate peptides. The negatively charged QDs recognize the change in net charge of the peptide upon phosphorylation. Despite its simple mechanism, this assay is sensitive and robust enough to be applied to the evaluation of protein kinase inhibitors.
机译：基于QD和荧光标记底物肽之间的FRET，开发了一种新型的蛋白激酶测定法。带负电荷的QD可识别磷酸化后肽净电荷的变化。尽管其机制简单，但该测定法灵敏且稳健，足以用于评估蛋白激酶抑制剂。

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  • 来源
    《Chemical Communications》 |2013年第49期|5592-5594|共3页
  • 作者

    Shujiro Shiosaki; Takanobu Nobori; Takeshi Mori; Riki Toita; Yuta Nakamura; Chan Woo Kim; Tatsuhiro Yamamoto; Takuro Niidome; Yoshiki Katayama;

  • 作者单位

    Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka,Nishi-Ku, Fukuoka 819-0395, Japan;

    Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka,Nishi-Ku, Fukuoka 819-0395, Japan;

    Department of Applied Chemistry, Faculty of Engineering, Kyushu University,744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan,Center for Future Chemistry, Kyushu University, 744 Motooka, Nishi-Ku,Fukuoka 819-0395, Japan;

    Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka,Nishi-Ku, Fukuoka 819-0395, Japan;

    Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka,Nishi-Ku, Fukuoka 819-0395, Japan;

    Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka,Nishi-Ku, Fukuoka 819-0395, Japan;

    Institute of Systems, Information Technologies and Nanotechnologies,203-1 Motooka, Nishi-Ku, Fukuoka 819-0385, Japan;

    Department of Applied Chemistry, Faculty of Engineering, Kyushu University,744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan,Center for Future Chemistry, Kyushu University, 744 Motooka, Nishi-Ku,Fukuoka 819-0395, Japan;

    Department of Applied Chemistry, Faculty of Engineering, Kyushu University,744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan,Center for Future Chemistry, Kyushu University, 744 Motooka, Nishi-Ku,Fukuoka 819-0395, Japan,International Research Center for Molecular Systems, Kyushu University,744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan,Center for Advanced Medical Innovation, Kyushu University, 744 Motooka,Nishi-Ku, Fukuoka 819-0395, Japan;

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  • 入库时间 2022-08-17 13:18:27

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