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A generalized strategy for immobilizing uniformly oriented membrane proteins at solid interfaces

机译:将均匀定向的膜蛋白固定在固体界面上的通用策略

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摘要

We have developed a method based on self-assembly of thiols on Au substrates to immobilize membrane proteins at interfaces. Using water soluble nitrilotriacetic acid (NTA)-terminated oligo(ethylene glycol) thiols, a histidine-tagged G protein-coupled membrane receptor (GPCR) was captured in a defined orientation with little nonspecific binding.
机译:我们已经开发了一种基于硫醇在Au底物上自组装的方法,以将膜蛋白固定在界面上。使用水溶性的次氮基三乙酸(NTA)末端的低聚(乙二醇)硫醇,组氨酸标记的G蛋白偶联膜受体(GPCR)以明确的取向被捕获,几乎没有非特异性结合。

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  • 来源
    《Chemical Communications》 |2013年第26期|2685-2687|共3页
  • 作者单位

    National Institute of Standards and Technology (NIST) Center for Neutron Research, Gaithersburg, MD 20899, USA Department of Materials Science and Engineering, University of Maryland,College Park, MD 20742, USA;

    National Institute of Standards and Technology (NIST) Center for Neutron Research, Gaithersburg, MD 20899, USA Department of Materials Science and Engineering, University of Maryland,College Park, MD 20742, USA;

    Materials Measurement Laboratory, NIST, Gaithersburg, MD 20899, USA;

    Materials Measurement Laboratory, NIST, Gaithersburg, MD 20899, USA;

    National Institute of Standards and Technology (NIST) Center for Neutron Research, Gaithersburg, MD 20899, USA;

    Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda,Maryland 20892, USA;

    Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda,Maryland 20892, USA;

    Institute for Bioscience and Biotechnology Research, NIST, Rockville, MD 20850,USA;

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  • 正文语种 eng
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  • 入库时间 2022-08-17 13:18:08

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