Asymmetric cooperativity in tandem hybridization of enantiomeric metal complex-tethered short fluorescent DNA probes

摘要

The complex [Ru(phen)_2(dppz)]~(2+)(phen = 1,10-phenanthroline, dppz = dipyrido[3,2-a:2',3'-c]phenazine) was attached to the 5' end of a short oligonucleotide to form conjugates, the A-isomer of which showed a high cooperativity during the recognition of the repetitive sequence, while the A-isomer did not.In chromosomal DNA there are a large number of repetitive sequences such as tandem repeats and palindromes.These sequences play important roles in genetic events.Telomeres,for example,which are the tandem repeat sequences located at the ends of linear chromosomes,would protect them from degradation and are related to apoptosis and tumour formation.