Expression, deleton and mutation of p16 gene in human gastric cancer

摘要

AIM To investigate the relationship between the expression of p16 gene and the gastric carcinogenesis depth of invasion and lymph node metastases, and to evaluate the deletion and mutation of exon 2 in p16 gene in gastric carcinoma. METHODS The expression of P16 protein was examined By streptavidin-peroxidase conjugate method 9S-P); the Deletion and mutation of p16 gene were respectively Examined by polymerase chain reaction (PCR) and Polymerase chain reaction single-strand conformation Polymorphism analysis (PCR-SSCP) in gastric carcinoma.