Effect of cryopreservation and phenylacetate on biological characters of adherent LAK cells from patients with hepatocellular carcinoma

摘要

Conclusion: A-LAK cells can be simply prepared by using PME, and showed a synergistic anti-tumor effect with the Combination of PA. Cryopreservation can increase the Immunoactivities of A-LAK cells from the patients with Hepatocellular carcinoma. Aim: To improve the preparation of adherent lymphokine- Activated killer(A-LAK)cells and to study the effects of Cryopreservation and phenylacetate(PA)on biological Characters of A-LAK cells. Methods: A-LAK cells were obtained from peripheral blood Mononuclear cells(PBMCs)of the patients with Hepatocellular carcinoma(HCC)by using L-phenylalanine Methyl ester(PME)to deplete immunosuppressive Monocytes.