Serum sIL-2R, TNF-α and IFN-γ in alveolar echinococcosis

摘要

AIM: To approach the relationship between alveolar echinococcosis (AE) pathology and level of sIL-2R,TNF-α and IFN-γ in sera and the significance of cytokines in development of AE. METHODS: After 23 patients with AE were confirmed by ELISA and ultrasound, their sera were collected and the concentrations of sIL-2R,TNF-α and IFN-γ were detected by double antibody sandwich. Twelve healthy adults served as controls. According to the status of livers of AE patients by ultrasound scanning, they were divided into 4 groups: P_2, P_3, P_4 groups and C group (control). Average of concentrations of sIL-2R, TNF-α and IFN-γ in homologous group was statistically analyzed by both ANOV and Newman-Keuls, respectively. RESULTS: The mean of SIL-2R in P_2 group was 97 +- 29, P_3: 226 +- 80, P_4: 194 +- 23 and control group (111 +- 30)x10~3u/L (P < 0.01). The mean of TNF-α in P_2 group was 1.12 +- 0.20, P_3: 3.67 +- 1.96, P_4: 1.30 +- 0.25 and control group 0.40 +- 0.19 μg/L (P < 0.01). The mean of IFN-γ in P_2 group was 360 +- 20, P_3: 486 +- 15, P_4: 259 +- 19 and control group: 16 +- 2 ng/L (P < 0.01). Judged by ANOV and Newman-Keuls, the mean concentrations of SIL-2R, TNF-α and IFN-γ had a significant difference among groups. Except for P_2 group, the mean sIL-2R between other groups of AE patients had a significant difference (P < 0.05). The mean of TNF-α concentration in P_3 group was the highest (P < 0.01). The mean of IFN-γ concentration in all patients was higher than that in control group (P < 0.01), but there was no difference between AE groups (P > 0.05). CONCLUSION: Low sIL-2R level indicates an early stage of AE or stable status, per contra, a progression stage. Higher level of TNF-α might be related to the lesion of liver. The role of single IFN-γ is limited in immunological defense against AE and it can not fully block pathological progression.