Relation of CagA seropositiity to cagPAI phenotype and histological grade of gastritis in patients with Helic0bacter py/ori infection

摘要

AIM: Infection with Helicobacter pylori(H pylori) possessing the cag pathogenicity island (PAI) has been associated with severe clinical outcome and CagA-antibody has been used to indicate cspPAI-positive infection. The aim of this study was to examine the accuracy of CagA seropositivity to indicate the virulence of the capPAI in Japan. METHODS: Sixty isolates of H pylori cultured from gastric biopsies were examined by polymerase chain reaction assays for the presence of cagA, cagE and VirD4. Anti-CagA IgG antibody in matching sera was tested by both ELISA and immunoblot assay. Histological grade of gastritis was graded according to the updated Sydney System. RESULTS: Amongst 53 patients infected with cagA+/ cagE+/ VirD4+ strain, 38 were CagA seropositive. There were four patients infected with strains possessing incomplete cagPAI. Two out of three patients with cagA+/ cagE-/ VirD4- infection were CagA seropositive, while a patient with cagA-/ cagE+/ VirD4+ infection was CagA seronegative. Accuracy of ELJSA to predict bacterial possession of cagA was 61.7% whereas 58.3% for cagEand VirD4. The immunoblot assay showed relatively higher sensitivity and showed better accuracy. The lower grade of gastric mucosal inflammatory infiltration was seen in false CagA-seronegative patients. CONCLUSION: Some serodiagnosis does not seem to have enough accuracy to indicate virulence of cagPAI, particularly in infection of strains with incomplete cagPAI. The degree of gastric mucosal inflammation may affect the results of CagA serodiagnosis.