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首页> 外文期刊>World Journal of Gastroenterology >Protective effect of curcumin against liver warm ischemia/reperfusion injury in rat model is associated with regulation of heat shock protein and antioxidant enzymes
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Protective effect of curcumin against liver warm ischemia/reperfusion injury in rat model is associated with regulation of heat shock protein and antioxidant enzymes

机译：姜黄素对大鼠肝缺血/再灌注损伤的保护作用与调节热休克蛋白和抗氧化酶有关

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AIM: To investigate the hypothesis that the protective effects of curcumin in hepatic warm ischemia/reperfusion (I/R) injury are associated with increasing heat shock protein 70 (Hsp70) expression and antioxidant enzyme activity. METHODS: Sixty Sprague-Dawley male rats were randomly divided into sham, I/R, C + I/R groups. The model of reduced-size liver warm ischemia and reperfusion was used. Curcumin (50 mg/kg) was administered by injection through a branch of superior mesenteric vein at 30 min before ischemia in C + I/R group. Five rats were used to investigate the survival during 1 wk after operation in each group. Blood samples and liver tissues were obtained in the remaining animals after 3, 12, and 24 h of reperfusion to assess serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver tissue NO_2~- + NO_3~-, malondialdehyde (MDA) content, superoxide dismutase (SOD), catalase (CAT), nitricoxide synthase (NOS) and myeloperoxidase (MPO) activity, Hsp70 expression and apoptosis ratio. RESULTS: Compared with I/R group, curcumin pretreatment group showed less ischemia/reperfusion-induced injury. CAT and SOD activity and Hsp70 expression increased significantly. A higher rate of apoptosis was observed in I/R group than in C + I/R group, and a significant increase of MDA, NO_2~- + NO_3~-and MPO level in liver tissues and serum transaminase concentration was also observed in I/R group compared to C + I/R group. Curcumin also decreased the activity of inducible NO synthase (iNOS) in liver after reperfusion, but had no effect on the level of endothelial NO synthase (eNOS) after reperfusion in liver. The 7 d survival rate was significantly higher in C + I/R group than in I/R group. CONCLUSION: Curcumin has protective effects against hepatic I/R injury. Its mechanism might be related to the overexpression of Hsp70 and antioxidant enzymes.
机译：目的：探讨姜黄素对肝温暖缺血/再灌注（I / R）损伤的保护作用与增加热休克蛋白70（Hsp70）表达和抗氧化酶活性有关的假设。方法：将60只Sprague-Dawley雄性大鼠随机分为假，I / R，C + I / R组。使用缩小的肝脏温暖缺血和再灌注模型。 C + I / R组缺血前30分钟，通过肠系膜上静脉分支注射姜黄素（50 mg / kg）。每组5只大鼠用于研究术后1周内的存活率。在再灌注3、12和24小时后，在其余动物中获得血样和肝组织，以评估血清丙氨酸氨基转移酶（ALT），天冬氨酸氨基转移酶（AST），肝组织NO_2〜-+ NO_3〜-，丙二醛（MDA）含量，超氧化物歧化酶（SOD），过氧化氢酶（CAT），一氧化氮合酶（NOS）和髓过氧化物酶（MPO）活性，Hsp70表达和凋亡率。结果：姜黄素预处理组与缺血再灌注组相比，缺血/再灌注损伤少。 CAT和SOD活性以及Hsp70表达明显增加。 I / R组的细胞凋亡率高于C + I / R组，肝脏组织中的MDA，NO_2〜-+ NO_3〜-和MPO水平显着升高，并且血清转氨酶浓度升高。 / R组与C + I / R组相比。姜黄素还降低了再灌注后肝脏中诱导型NO合酶（iNOS）的活性，但对肝脏再灌注后内皮型NO合酶（eNOS）的水平没有影响。 C + I / R组的7 d生存率明显高于I / R组。结论：姜黄素对肝I / R损伤具有保护作用。其机制可能与Hsp70和抗氧化酶的过表达有关。

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