Effects of the cysteinyl leukotriene receptor antagonists pranlukast and zafirlukast on tracheal mucus secretion in ovalbumin-sensitized guinea-pigs in vitro

摘要

We investigated the inhibitory effects of the cysteinyl leukotriene (CysLT_1) receptor antagonists, pranlukast and zafirlukast, on ~35SO_4 labelled mucus output, in vitro, in guinea-pig trachea, induced by leukotriene D_4(LTD_4) or by antigen challenge of sensitized animals. Agonists and antagonist were administered mucosally, except in selected comparative experiments where drugs were administered both mucosally and serosally to assess the influence of the epithelium on evoked-secretion. LTD_4 increased ~35SO_4 output in a concentration-related manner with a maximal increase of 23 fold Above controls at 100 μM and an approximate EC_50 of 2 μM.