Characterization of high affinity neurotensin receptor NTR1 in HL-60 cells and its down regulation during granulocytic differentiation

摘要

1. We investigated responses to neurotensin in human promyelocytic leukaemia HL-60 cells. 2. Neurotensin increased the cytosolic calcium concentration ([Ca~2+]_I) in a concentration-dependent manner and also produced inositol 1, 4, 5-trisphosphate (InsP_3). 3. Among the tested neurotensin analogues, neurotensin 8-13, neuromedin-N, and xenopsin also increased [Ca~2+]_I whereas neurotensin 1-11 and neurotensin 1-8 did otelicit detectable responses.