Identification of novel polymorphisms within the promoter region of the human β_2 adrenergic receptor gene

摘要

By screening the 1470 bp 5' to the start codon of the humanβ_2 adrenergic receptor gene, we have identified a total of eight polymorphisms (-20 T→C, -47 T→C, -367 T→C, -468 C→G, -654 G→A, -1023 G→A, -1343 A→G and -1429 T→A c.f. β_2 adrenergic receptor start codon). Transient transfection of 5' flanking deletion luciferase reporter constructs demonstrated the Majority of activity of the humanβ_2 adrenergic gene 5' flanking region to be present within a 549 bp Fragment immediately upstream from the start codon.