Development of a simple enzyme-linked immunosorbent assay for the detection of autoantibodies in anti-p200 pemphigoid

摘要

Background Anti-p200 pemphigoid is a subepidermal blistering skin disease characterized by autoantibodies against a 200-kDa protein (p200) of the dermal–epidermal junction. The laminin 1 chain has recently been identified as target antigen in this disease and the C-terminus was described as an immunodominant region of laminin 1. Diagnosis of anti-p200 pemphigoid requires detection of serum IgG at the dermal side of 1?mol?L?1 salt-split skin by indirect immunofluorescence microscopy and labelling of a 200-kDa protein by Western blotting of dermal extract. However, preparation of dermal extract is not widely available, limiting the possibility of diagnosing this disease to a few laboratories.