Optimization of Nutrient Medium Containing Agricultural Waste for Xylanase Production by Bacillus pumilus B20

摘要

We aimed to optimize a nutrient medium containing agricultural waste for xylanase production by Bacillus pumilus B20. Xylanase production with ligno-cellulosic material was optimized in two steps using DeMeo's fractional factorial design. A 3.4-fold increase in xylanase production (313.3 U/mL) was achieved using the optimized culture medium consisting of (g/L): K_2HPO_4, 2; MgSO_4-7H_2O, 0.3; CaCl_2-2H_20,0.01; NaCl, 2; peptone, 5; yeast extract, 4; and wheat bran, 50. B. pumilus B20 produced a high level of xylanase, which may have potential industrial application.