Durchflusszytometrie zum Auffinden verbesserter und neuer Enzyme

摘要

Flow cytometry screening systems in emulsions offer a throughput (up to 108) that would enable novel strategies in directed protein evolution (e. g. with high mutational loads) and enzyme discovery in metagenomes (hit rates < 1 in 105). A main challenge which hampers a routinely use of flow cytometry is the lack of suitable screening assays. This article highlights the potential of flow cytometry to improve a monooxygenase with high mutational loads and to discover novel enzymes in metagenomes.