Role of Differential Expression of sdh 1-1 and sdh 1-2 Genes in Alteration of Isoenzyme Composition of Succinate Dehydrogenase in Germinating Maize Seeds

摘要

A probable mechanism of alteration of the isoenzyme composition of succinate dehydrogenase (SDH) due to differential expression of genes encoding subunit A was considered. The alteration of SDH activity during maize seed germination was investigated, and its maximal activity on day 4-5 of germination was found. The alteration of the sdh 1-1 and sdh 1-2 gene expression level during maize seed germination was evaluated using the quantitative polymerase chain reaction method. The presence of four forms of the studied enzymes, providing multiple SDH functions was found in maize inflorescence using electrophoresis in poly-acrylamide gel.