The Expression of Genes Encoding the Voltage-Dependent L-type Ca~(2+) Channels in Proliferating and Differentiating C2C12 Myoblasts of Mice

摘要

Voltage-dependent L-type Ca+ channels of the C2C12 line myoblasts of mice have been studied at the stage of proliferation and 24 h after the beginning of differentiation. The expression of genesCacnals, Cacnalc, Cacnald, and Cacnalf which encode channel forming subunits a,S, otiC a,D, and a,F, respectively, has been assessed. The expression of genes Cacna2d and Cacnlg, which encode the a2, 5, and y regulatory subunits, has been studied as well. For the first time, the expression of Cacnald, which is typical for nerve cells, has been revealed in proliferating myoblasts, whereas in differentiating mononuclear myoblasts the expression of this gene was significantly decreased. On the contrary, the low level of expression of CacnalS, which encodes the specific cqS channel forming subunit of skeletal muscles, has been observed in proliferating myoblasts, whereas in differentiating mononuclear myoblasts it has been shown to increase multifold. No considerable changes in expression of Cacna2d and Caen Ig have been revealed in proliferating and differentiating myoblasts. No traces of expression ofCacnalc and Cacna //have been revealed in myoblasts.