Metal-ion catalyzed oxidation affects fibrinogen activity on platelet aggregation and adhesion

摘要

Exposure of fibrinogen to the Fr~3+/ascorbate oxidative system resulted in structural modifications and altered functionality of the glycoprotein. The overnight treatment of fibrinogen by oxidants caused a 20-fold increase of carbonyl content with respect to the native protein. Formation of dityrosines as well as loss of tryptophan following fibrinogen oxidation were observed. The occurrence of conforma- tional changes of the fibrinogen molecule as a consequence of the oxidative treatment was also established.