Effects of Recombinant Protein Expression on Green Fluorescent Protein Diffusion in Escherichia coli

摘要

Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of greennfluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins:ncytoplasmic maltose binding protein (42 kDa), tau-40 (45 kDa), R-synuclein (14 kDa), or calmodulinn(17 kDa). The GFP diffusion coefficient remains constant regardless of the type of coexpresseed protein andnwhether or not the coexpressed protein was induced.We conclude that expression of these soluble proteins hasnlittle to no effect on the diffusion of GFP. These results have implications for the utility of in-cell nuclearnmagnetic resonance spectroscopy