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Biological and molecular characterization of a putative new sobemovirus infecting Imperata cylindrica and maize in Africa

机译:推定的新的猪瘟病毒感染非洲白茅和玉米的生物学和分子特征

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A new virus was isolated from both the grass Imperata cylindrica and maize plants that had yellow mottle symptoms in Burkina Faso, West Africa. The virus has isometric particles ca. 32 nm in diameter. The experimental host range was restricted to Rottboellia exaltata. Virions were isolated from leaves of systemically infected maize plants. Koch’s postulates were completed by mechanically inoculating uninfected Imperata or maize with either purified virus or sap from infected Imperata plants. Virion preparations were used to produce a specific polyclonal antiserum, and an enzyme-linked immunosorbent assay test was set up. The full genome of the virus was sequenced, and it comprised 4,547 nucleotides. Phylogenetic studies indicated that the virus is closely related to rice yellow mottle virus, a sobemovirus that infects monocotyledons in Africa, and is more distantly related to cocksfoot mottle virus, another sobemovirus that infects monocotyledons. Although the virus can infect R. exaltata experimentally, it differs from Rottboellia yellow mottle virus, a member of a tentative species of the genus Sobemovirus that also infects monocotyledons in Africa. Particle morphology, serological properties, genomic organization, and phylogenetic analysis are all consistent with assignment of the new virus to the genus Sobemovirus. The name Imperata yellow mottle virus is proposed.
机译:在西非布基纳法索,从草白茅和玉米植物中分离出一种新病毒,这些病毒具有斑驳症状。该病毒约有等距颗粒。直径32纳米。实验寄主范围仅限于红腹轮虫。从系统感染的玉米植物的叶子中分离病毒体。 Koch的假设是通过用未感染的Imperata或玉米机械接种来自感染的Imperata植物的纯化病毒或汁液来完成的。使用病毒颗粒制剂产生特定的多克隆抗血清,并建立了酶联免疫吸附试验。对该病毒的完整基因组进行了测序,它包含4,547个核苷酸。系统发育研究表明,该病毒与感染非洲单子叶植物的米黄色斑驳病毒密切相关,而与另一种感染单子叶植物的so毛斑驳病毒密切相关。尽管该病毒可以通过实验感染极高的R. exaltata,但它不同于Rottboellia黄色斑驳病毒,后者是Sobemovirus属的一种试探性物种,也感染了非洲的单子叶植物。颗粒形态,血清学特性,基因组组织和系统发育分析均与将新病毒分配给Sobemovirus属一致。建议命名白茅黄斑驳病毒。

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