Consolidated Bioprocessing of Sugarcane Bagasse to Microbial Oil by Newly Isolated Oleaginous Fungus: Mortierella wolfii

摘要

The crucial need for alternative oil sources due to increasing energy demand has motivated research to find alternativesresources. In the current study, different fungal groups were isolated from diverse Egyptian localities for oil productionscreening as well as sugarcane bagasse management. A total of 499 fungal isolates were recovered from soil and dungsamples; one isolate was identified as Mortierella wolfii AH12 using morphological and molecular techniques and wasdemonstrated to produce high lipid concentrations. Taguchi design was used to obtain higher lipid productivity. Accordingly,dry biomass, total lipids, and lipid percentage were 3.81 g l~(−1), 1.57 g l~(−1), and 41.2%, respectively, under the optimized cultureconditions: 40 g glucose, 5 g peptone, pH 5 at 30 0°C for 10 days. Non-pretreated sugarcane bagasse was not only utilized asan alternative carbon source, but it was also an effective source of nutrients for mold sprouting and lipid biosynthesis usingsolid-state fermentation (SSF) technique. Promising fatty acid profile was deduced, where palmitic (PA) and oleic acid (OA)were predominant. Moreover, unsaturated fatty acids (USFAs) as linoleic acid (LA), Gamma linolenic acid (GLA), Dihomogamma linolenic acid (DGLA), arachidonic acid (ARA) were present in low quantities according to GC–MS analysis. Thestudy offers a promising result that serves in the management of agricultural waste, converting it into lipids with a promisingprofile which could be considered as a precursor for biodiesel.