SPECTROPHOTOMETRIC DETERMINATION OF AMIKACIN SULPHATE VIA CHARGE TRANSFER COMPLEX FORMATION REACTION USING TETRACYANOETHY LENE AND 2,3-DICHLORO-5,6-DICYANO-1,4-BENZOQUINONE REAGENTS

摘要

A spectrophotometric method based on the complex formation reaction has been described for the determination of amikacin sulphate as n-donor with terecyanoethylene (TCNE) and 2,3-dichloro-5,6-dicyano-l,4-benzoquinone (DDQ) reagents as π-acceptors to give a colored complex species in aqueous solution which can absorb maximally at 330 and 340 nm, respectively. Beer's law is obeyed in the concentration ranges 0.4-6.8 and 0.8-20.0 μg/ml with high apparent molar absorptivities of 9.49 × 10~4 and 1.91 × 10~4 and limits of detections are 0.06 and 0.18 μg/ml, whereas the average recoveries are 96.54% and 99.47% when using TCNE and DDQ, respectively. The relative standard deviation is ≤ 4. Application of the proposed method to commercial pharmaceutical injuctions is presented.