Induction of ethoxyresorufin-O-deethylase (EROD) activity in the liver of black porgy (Acanthopagrus schlegeli) exposed to benzo(a)pyrene

摘要

This paper studied the induction kinetics of the enzyme 7-ethoxyresorufin O-deethylase in the liver of the marine fish, Acanthopagrus schlegeli following the exposure to benzo (a)pyrene for up to 14 days at concentrations of 0.5,1.0,2.0,5.0 μg l~(-1). The results demonstrated a dose-response relationship between hepatic enzyme activities induction and benzo(a)pyrene concentration. Hepatic enzyme activities were significantly induced by the second day with exposure to the lower concentrations of benzo(a)pyrene (0.5 μg l~(-1) and 1.0 μg l~(-1)), and at 12 hours of exposure for the higher concentrations (2.0 l~(-1) and 5.0 μg l~(-1)). After 7 days depuration, the activities of the enzyme offish returned to the control level. This indicated that while benzo(a)pyrene has an effect on the hepatic enzyme activity, the fish can eliminate a major part of BaP metabolites.