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首页> 外文期刊>Aquaculture Research >Effect of dietary conjugated linoleic acid levels on growth performance, muscle fatty acid profile, hepatic intermediary metabolism and antioxidant responses in genetically improved farmed Tilapia strain of Nile tilapia Oreochromis niloticus
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Effect of dietary conjugated linoleic acid levels on growth performance, muscle fatty acid profile, hepatic intermediary metabolism and antioxidant responses in genetically improved farmed Tilapia strain of Nile tilapia Oreochromis niloticus

机译:日粮共轭亚油酸水平对尼罗罗非鱼罗非鱼基因改良养殖罗非鱼生长性能,肌肉脂肪酸谱,肝中间代谢和抗氧化反应的影响

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An 8-week feeding trial was conducted to determine the effect of dietary conjugated linoleic acid (CLA) on growth performance, muscle fatty acid profile, hepatic intermediary metabolism and antioxidant responses in genetically improved farmed Tilapia (GIFT) strain of Oreochromis niloticus (initial body weight: 42.6?±?0.4?g, mean?±?standard deviation). Three replicated groups of GIFT strain of Nile tilapia were hand-fed to satiation, twice a day, with the diets in which CLA oil, containing mainly the bioactive cis-9, trans-11 and trans-10, cis-12 isomers, was included at 0 (control), 0.5%, 1%, 1.5%, 2% and 2.5%, respectively, at the expense of fish oil to maintain the constant lipid and energy levels. Growth performance and feed utilization showed no significant differences among the treatments (P?>?0.05). The dietary inclusion of CLA modified total percentages of the main groups of fatty acids. Increasing saturated fatty acid content and reduced mono-unsaturated fatty acid contents in muscle were observed with increasing dietary CLA inclusion (P??0.05). Dietary CLA supplementation resulted in the significant increase in the trans-10, cis-12 and cis-9, trans-11 CLA isomers in muscle (P?
机译:进行了为期8周的喂养试验,以确定日粮共轭亚油酸(CLA)对改良遗传养殖的罗非鱼(GIFT)尼罗罗非鱼(初始体)的生长性能,肌肉脂肪酸谱,肝中间代谢和抗氧化反应的影响重量:42.6±±0.4μg,平均值±标准偏差)。每天两次将三组复制的尼罗罗非鱼的GIFT菌株人工喂养至饱腹感,其中的饮食中主要含有生物活性的顺式9,反式11和反式10,顺式12异构体的CLA油。分别以0(对照组),0.5%,1%,1.5%,2%和2.5%的含量添加鱼油,以维持恒定的脂质和能量水平为代价。各处理之间的生长性能和饲料利用率没有显着差异(P≥0.05)。在饮食中添加CLA可以改变主要脂肪酸组的总百分比。随着饮食中CLA含量的增加,肌肉中饱和脂肪酸含量增加,单不饱和脂肪酸含量降低(P <0.05)。随着饮食中CLA水平的升高,总n-3脂肪酸和总多不饱和脂肪酸趋于下降(P 0.05),但各处理之间n-6脂肪酸无显着差异(P <> 0.05)。饮食中补充CLA导致肌肉中反式10,顺式12和顺式9,反式11的CLA异构体显着增加(P 0.05),并且还显着影响了几种肝酶活性,例如琥珀酸脱氢酶,乳酸脱氢酶,苹果酸脱氢酶,脂蛋白脂肪酶和肝脂肪酶活性(P≤0.05)。在补充了CLA的日粮中,鱼的超氧化物歧化酶和谷胱甘肽过氧化物酶活性降低,丙二醛水平下降(P <0.05),表明日粮中添加CLA对这种鱼具有强大的抗氧化作用。我们的研究是第一个涉及饮食中添加CLA对鱼类肝脏中间代谢和抗氧化反应的影响的报告,可将其用作鱼类营养和生理状况的指标。

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